Research Paper Volume 15, Issue 3 pp 718—733
Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts
Figure 1. Effect of osteoporosis and BMP9 on IL-6 Expression in MSCs. (A) Rat serum level of IL-6 was measured with ELISA. (B) Endogenous IL-6 mRNA expression was measured with real-time PCR. (C) Effect of BMP9 on IL-6 mRNA expression was measured with real-time PCR. (D) Effect of BMP9 on IL-6 protein level was assayed with western blot. (E) quantification result of western blot assay shows the effect of BMP9 on IL-6 protein level. (F) The effect of recombinant adenoviruses on the target protein level were measured with western blot assay (upper panel show the infection of recombinant adenoviruses in MEFs). (* P < 0.05, ** P < 0.01).