Research Paper Volume 15, Issue 1 pp 37—52

Figure 6. Pharmacological modulation of RORα regulates human choroidal endothelial cell angiogenic function. (A) Relative mRNA expression of RORα in human choroidal endothelial cell (hCEC), human retinal microvascular endothelial cell (hRMEC), mouse brain smooth muscle cell (mSMC) and mouse whole retina (mRetina), measured with quantitative RT-PCR and normalized to housekeeping gene GAPDH (human) and Gapdh (mouse) respectively. n = 3/group. (B) HCECs were treated with RORα inverse agonist (SR3335), agonist (SR1078), or DMSO vehicle control. MTT assay was performed to evaluate cell viability and proliferation. Cell growth was calculated as fold change of relative absorbance normalized to the values at 0 hr. n = 3/group. (C, D) Quantification analysis (C) and representative images (D) of hCEC migration assay. Cells were grown to confluence and treated with SR3335, SR1078, or DMSO vehicle control. Mitomycin was used to inhibit cell proliferation. A scratch wound was generated in the cells. Cell migration were measured after 24 hr and quantified as new cell coverage areas normalized by the original wound areas. n = 4/group. Scale bar: 250 μm. ^*P ≤ 0.05; ^**P ≤ 0.01; ^***P ≤ 0.001; ^****P ≤ 0.0001.