Figure 6. Effects of Sirt1 on luteolin in alleviating dry eye symptoms in DEDC mice. (A) Phenol red cotton thread testing mouse tears. (B) Sodium fluorescein staining. (C) Goblet cell staining. (D) Western blotting detects protein expression of AC-NF-κB, NF-κB and GSDMD-N, GSDMD in tear fluid. Data analysis was performed with mean ± SEM. ##p < 0.01 compared with blank group; *p < 0.05, **p < 0.01 compared with model group, n = 9.