Research Paper Volume 15, Issue 1 pp 21—36

Figure 3. fbxc-58 mediates dietary restriction effects on mitigating muscle aging. (A) The number of body bends in N2 L4440 RNAi (N2 L4440) (n = 15), eat-2 L4440 RNAi (eat-2 L4440) (n = 26), and eat-2 fbxc-58 RNAi (eat-2 fbxc-58) (n = 20) at day 10 of adulthood. (B) MVs of N2 L4440 RNAi (N2 L4440) (n = 15), eat-2 L4440 RNAi (eat-2 L4440) (n = 15), and eat-2 fbxc-58 RNAi (eat-2 fbxc-58) (n = 19) at day 10 of adulthood. (C) The morphological categories of mitochondria are defined as follows. Images showing most of the long interconnected mitochondrial networks were classified as tubular, and images showing most of the short mitochondria or sparse globular mitochondria were classified as fragmented. Mitochondrial morphology was examined in PD4251 and eat-2(ad1116); PD4251 strain that emits fluorescence from mitochondria due to GFP expressed in mitochondria. (Left) Representative images of N2 L4440 RNAi (N2 L4440), eat-2 L4440 RNAi and eat-2 fbxc-58 RNAi at day 8 of adulthood. (Right) Qualitative analysis of mitochondrial morphology in N2 L4440 RNAi (N2 L4440), eat-2 L4440 RNAi (eat-2 L4440) and eat-2 fbxc-58 RNAi (eat-2 fbxc-58) at day 8 of adulthood. Bars represent the proportion of worms with fragmented mitochondria. (D) A schematic diagram of fbxc-58 mediating DR-induced effects. Error bars represent SEM. ^*p < 0.05, ^**p < 0.01, ^****p < 0.0001; unpaired t test.