Research Paper Volume 15, Issue 1 pp 21—36

Figure 1. Screen to uncover the innate immune signal mediating the dietary restriction (DR) effect. (A) Venn diagram depicting the genes overlapping between genes upregulated in DR-worms and upregulated in pathogen infected worms. (B) Relative expression level of each F-box gene between wild-type (N2) and eat-2(ad1116) mutant strains at day 2 of adulthood. (C) Relative expression levels of fbxc-58 mRNA in N2 on ad libitum (AL) and low density of food (LDF) condition at day 2 of adulthood. (D) Relative expression level of fbxc-58 in L4440 (L4440 RNAi N2) and fbxc-58 (fbxc-58 RNAi N2) at day 3 of adulthood. (E) (I) Schematic diagram for Pseudomonas aeruginosa (PA14) infection assay. (II) The survival rate of N2 L4440 (L4440 RNAi N2) and N2 fbxc-58 (fbxc-58 RNAi N2) in PA14 infection for 24 h at day 9 of adulthood. All of relative mRNA levels were determined by RT-PCR by three times independent experiments, normalized to act-3. Error bars represent SEM. Abbreviation: ns: not significant, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001; unpaired t test.