Figure 4.WTX is a direct target of miR-874-3p. (A) The four-way Venn diagram reveals the numbers of overlapping miRNAs obtained using four publicly available bioinformatics algorithms and the microarray-based VDAC1 signature. (B) RT-qPCR was used to detect the relative expression of miR-874-3p in normal breast cells and breast cancer cells. (C) RT-qPCR was used to detect the expression in 18 breast cancer tissues and adjacent paired normal breast tissue samples. (D) Correlation between miR-874-3p levels and VDAC1 levels in 18 breast cancer tissues. (E) Nucleotide predicted miR-874-3p-binding site in the VDAC1 mRNA 3′-UTR. (F) MCF-7 and BT549 cells were transfected with reporter plasmids containing WT-pmir-VDAC1 or MUT-pmir-VDAC1 and miR-874-3p mimic or miR-874-3p mimic NC, and luciferase activity was detected. Data represent the means ± S.D. **P < 0.01.
