Research Paper Volume 14, Issue 24 pp 10050—10066

Figure 4. SGOL2 stabilized RAB1A via a ubiquitination-dependent pathway. (A) SDS-PAGE of the control group (left) and shSGOL2 group (right) was stained by Coomassie Blue Staining Solution. (B) Cell lysates were subjected to Co-IP with specific antibodies to examine the interaction between SGOL2 and RAB1A. (C) Detect RAB1A expression as in A in DU145 and LNCaP. (D) At 48h post-infection, WB analysis of RAB1A expression in shSGOL2 cell lines treated with CHX for 0, 2, 4, 8h compared to the control group. (E) Treated with protease inhibitor MG132 for 8h, cells were lysed and subjected to WB for RAB1A expression. (F) WB analysis for IPs performed with anti-ubiquitin antibody to detect SGOL2-mediated RAB1A ubiquitination.