Research Paper Volume 14, Issue 24 pp 10009—10026
Role of m6A modification and novel circ_0066715/ miR-486-5p/ ETS1 axis in rheumatoid arthritis macrophage polarization progression
Figure 4. circ_0066715 regulates the miR-486-5p/ETS1 axis. (A) ETS1 binding site to miR-486-5p. (B) Luciferase reporter. **P < 0.01. (n=3, ANOVA with Bonferroni’s post test). (C) ETS1 mRNA expression in miR-486-5p overexpressing RA-FLS cells. **P < 0.01. (n=3, ANOVA with Bonferroni’s post test). (D, E) Expression of ETS1 protein in miR-486-5p overexpressed RA-FLS cells was performed by western blotting. **P < 0.01. (n=3, ANOVA with Bonferroni’s post test). (F) ETS1 expression in FLS cells (IF,×200). (G) IL-4 expression in FLS cells (IF,×200). (H) TNF-α expression in FLS cells (IF,×200).