Research Paper Volume 14, Issue 24 pp 9908—9923

Figure 2. Effects of fat body UAS-apoLpp^RNAi on arrhythmia and lipid metabolism. (A) Climbing index of fat body UAS-apoLpp^RNAi in 14- and 35-days-old flies. N = 50, see the “Materials and methods” section to calculate the climbing index. (B) Whole-body TG levels in 14- and 35-days-old flies by UAS-apoLpp^RNAi in the fat body. N = 5. Error bars represent three independent replicates. (C) Body weight of 14- and 35-days-old flies with UAS-apoLpp^RNAi in the fat body. N = 5, values are expressed as the body weight of 5 flies and measured in triplicate. (D) Whole-body apoLpp mRNA expression levels in 14- and 35-days-old flies for fat body UAS-apoLpp^RNAi. GAPDH was used to normalize these values, N = 10. (E–G) Heart rate, cardiac cycle, and arrhythmia index of 14- and 35-days-old flies with fat body UAS-apoLpp^RNAi. N = 30. (H) M-mode traces (8 s) prepared from high-speed movies of intact flies. All P-values are from student t-tests, all values are expressed as mean ± SEM, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.