Research Paper Volume 15, Issue 8 pp 2877—2890

Figure 5. LINC01296 interacted with miR-324-3p and repressed its expression in CMM. (A) A schematic diagram used to search the target miRNAs of LINC01296 in three databases. (B) qRT-PCR assay confirmed the relative expression of three candidate miRNAs of LINC01296 in 30 paired CMM tissues compared with adjacent non-cancerous tissues. (C) miR-324-3p expression level in CMM cell lines and human epidermal melanocytes, adult cell line (HEMa-LP) were detected by qRT-PCR analysis. (D) Schematic illustration of the predicted binding sites between LINC01296 and miR-324-3p, and mutation of potential miR-324-3p binding sequence in LINC01296. Relative luciferase activities of wild type (WT) and mutated (MUT) LINC01296 reporter plasmid in human embryonic kidney (HEK) 293T cells co-transfected with miR-324-3p mimic. (E) The relative expression of miR-324-3p in M21 and A2058 cells transfected with knocking down LINC01296 by qRT-PCR analysis. (F) The relative expression of miR-324-3p in M21 and A2058 cells transfected with miR-136-5p mimic by qRT-PCR analysis. (G) The relative expression of LINC01296 in M21 and A2058 cells transfected with miR-136-5p mimic by qRT-PCR analysis. All of data were analyzed from three independent experiments. *P < 0.05, ** P < 0.01, ^ns P>0.05.