Research Paper Volume 14, Issue 22 pp 8944—8969
JAK2/STAT3 pathway mediates neuroprotective and pro-angiogenic treatment effects of adult human neural stem cells in middle cerebral artery occlusion stroke animal models
Figure 8. Roles of JAK2/STAT3 signaling pathway in pro-angiogenic effects of ahNSCs. (A) HUVECs were challenged by OGD for 1 h and then incubated in IM, NSC-CM, or NSC-CM with AG490 (50 μM) for 24 h. After 24 h, the total tube length was measured and compared (n = 3 per group). Scale bar = 100 μm. Mean ± SD. ** P < 0.01, *** P < 0.001. (B) HUVECs challenged by OGD for 1 h were cultured in IM, NSC-CM, or NSC-CM with AG490 (50 μM) for 24 h and allowed to migrate through. After 24 h, relative ratio of migrated HUVECs was measured and compared. Scale bar = 100 μm. Mean ± SD. **P <0.01; ***P <0.001.