Research Paper Volume 14, Issue 22 pp 8944—8969

Figure 1. Significant therapeutic effects of ahNSCs for ischemic stroke. (A) In vivo experimental procedure was illustrated. (B) Left. Representative images of TTC staining at 28 d after ahNSCs transplantation (n = 3 for the control group, n = 7 for the HBSS group, n = 9 for the NSC group). The black dashed lines denote the atrophic regions of brains. Right. Brain atrophy volumes (%) were calculated and compared. Mean ± SEM. * P < 0.05; ** P < 0.01. (C) Rotarod test measured time to fall from rotating rotarod which accelerated from 5 to 40 rpm for 180 s. Each day, four latency times were averaged for each animal and compared (n = 7 for the control group, n = 6 for the HBSS group, n = 6 for the NSC group). Mean ± SEM. * P < 0.05; ** P < 0.01 at 28 d after ahNSCs transplantation. (D) Memory function, latency time to avoid pain stimuli using space clues, was analyzed by passive avoidance test (n = 6 for the control group, n = 15 for the HBSS group, n = 17 for the NSC group). Mean ± SEM. * P < 0.05; ** P < 0.01; ***P <0.001 at 28 d after ahNSCs transplantation. (E, F) In adhesive-removal test, contact (E) and removal (F) time until animals feel the sensation of adhesion and take off sticker by using their forepaws, respectively, was accessed (n = 13 for the control group, n = 23 for the HBSS group, n = 24 for the NSC group). Mean ± SEM. * P < 0.05; ** P < 0.01; ***P <0.001 at 28 d after ahNSCs transplantation.