Research Paper Volume 14, Issue 22 pp 9264—9279
CD229 interacts with RASAL3 to activate RAS/ERK pathway in multiple myeloma proliferation
Figure 6. CD229 binds to RASAL3 in a phosphorylated manner after self-activation. (A) Co-IP assay detected that the phosphorylation of CD229 and the expression of RASAL3 were decreased after Dasatinib treatment in CD229-OE MM cells. (B) Schematic diagram of the two co-culture experiments. (C, D) Representative confocal images for PKH26 and p-ERK revealed that higher p-ERK levels were observed in the directly mixed co-culture of WT MM cells than the co-cultured cells. The data of experiments represent Mean±SD from at least three independent experiments. ***p < 0.001.