Research Paper Volume 14, Issue 21 pp 8856—8875
Polydatin administration attenuates the severe sublesional bone loss in mice with chronic spinal cord injury
Figure 7. PLD promoted osteoblastogenesis in bone marrow progenitor cells generated from chronic SCI mice. (A) The number of osteoblast-like cells (CFU-F) and the number of mineralized nodules (CFU-OB) in BMSC cultures were identified by ALP staining and von Kossa staining, respectively. (B) Alizarin red staining was used to evaluate BMSC calcification in the presence or absence of OIM of in groups. (C) Quantitative analysis of the ALP activity assay. (D) RUNX2, OCN, ALP, OPG and RANKL mRNA levels, and OPG/RANKL ratio in the osteoblast-like cells. mRNA levels were determined by qRT-PCR analysis. Data are expressed as mean ± S.D.; n=4 per group; *P < 0.05, vs. the Sham group; #P < 0.05, vs. the SCI group.