Research Paper Volume 14, Issue 21 pp 8839—8855

Figure 1. lncRNA HHIP-AS1 and HHIP are continuously down-regulated during osteogenic differentiation of BM-MSCs. Up-regulated or down-regulated (A) lncRNAs and (B) mRNAs were analyzed by Venn diagram during osteogenic differentiation at days 0, 7 and 14 of osteogenic differentiation of BM-MSCs. Heatmap of (C) lncRNA and (D) mRNA changes at days 0, 7 and 14 of osteogenic differentiation of BM-MSCs. (E) FISH showing that lncRNA HHIP-AS1 was expressed in the nucleus and cytoplasm of BM-MSCs at day 7 of osteogenic differentiation of BM-MSCs. mRNA expression level of (F) lncRNA HHIP-AS1 and (G) HHIP during osteogenic differentiation. (H) Protein expression level of HHIP, RUNX2, and osteocalcin during osteogenic differentiation of BM-MSCs. The histogram data for each group are the average of three independent replicates; bars represent standard deviation; *P < 0.05, **P < 0.01, ***P < 0.001; Scale bar = 10 μm.