Research Paper Volume 14, Issue 21 pp 8645—8660

Figure 2. SCF^{Cyclin F} does not regulate USP7 protein levels. (A) HCT116 cells treated with either DMSO or MLN4924 (3 μM) for 5 h were lysed, and immunoblotted as indicated. β-Actin was the loading control. Asterisk denotes non-specific band. (B) HEK-293T cells were transfected with an empty vector or Flag-HA-Cyclin F for 48 h, lysed, and immunoblotted as indicated. (C) HeLa cells treated with DMSO, etoposide (10 μM) or MG132 (10 μM) for 4 h were lysed, and immunoblotted as indicated. Asterisk denotes non-specific band.