Research Paper Volume 14, Issue 22 pp 8886—8899

Figure 2. In vitro effects of Con-PGK1 and PEP-1-PGK1 on H₂O₂-induced oxidative stress in HT22 cells. (A) The optimal concentration of Con-PGK1 and PEP-1-PGK1 is assessed by measurements of cell viability using a water-soluble tetrazolium salt-1 assay 1 h after H₂O₂, Con-PGK1, or PEP-1-PGK1 treatment. (B) Survived cells, (C) ROS formation, and (D) DNA fragmentation are visualized by 5-CFDA AM, DCF, and TUNEL staining, respectively, 1 h after 200 μM H₂O₂, 5.0 μM Con-GPK1, or 5.0 μM PEP-1-PGK1 treatment. Scale bar = 50 μm. (B–D) The intensities of 5-CFDA AM-, DCF-, and TUNEL-stained structures were spectrophotometrically measured. (A–D) Data are analyzed by a one-way analysis of variance, followed by a Bonferroni’s post-hoc test (^ap < 0.05, significantly different from the control group; ^bp < 0.05, significantly different from the vehicle-treated group). The bar graph represents the mean ± standard deviation.