Research Paper Volume 14, Issue 20 pp 8321—8345

Figure 8. JPYS improved decreased cell viability, increased cell apoptosis caused by cyclophosphamide (CTX) in vitro. JPYS-containing serum inhibited loss of cells viability induced by CTX. (A) Cells were treated with CTX (20, 40, and 60 μg/ml) for 24 h; (B) Cells were treated with JPYS-containing serum (2, 4, 8 %); (C) Cells were treated with CTX (20 μg/ml) for 24 h, then treated with JPYS-containing serum (2, 4, 8 %); (D) Hoechst 33258 staining was used to detect the apoptosis and counted the percentage of apoptotic cells; (E) Cell apoptosis was measured by fow cytometry and counted the percentage of apoptotic cells: provided 2-dimensional graphical representations of PI/annexin V-FITC plots. ‘Early apoptosis’ was defifined as cells positive for annexin V-FITC only. ‘Late apoptosis’ was defifined as cells positive for annexin V-FITC and PI. ‘Necrosis’ was defifined as cells positive for PI only; (F) Cell apoptosis was observed by electron microscope pictures (10,000×), the scale bars represents a length of 2 μm on cells respectively. Data are shown as mean ± SD. *p < 0.05 versus control group, ^#p < 0.05 versus CTX group, ^△p < 0.05 versus CTX+ JPYS (2 %) group, ^▲p < 0.05 versus CTX+ JPYS (4 %) group. (n=3).