Research Paper Volume 14, Issue 18 pp 7443—7454
Different transcriptional profiles of human embryonic stem cells grown in a feeder-free culture system and on human foreskin fibroblast feeder layers
Figure 8. Validation of gene-relative changes through real-time RT-PCR. Fold changes of the gene expression in 3-line hESCs (NF4, NF5, P096) were calculated in a feeder-free culture system vs on human foreskin fibroblast feeder layers. Data was presented as mean values ± SD (n=3). The significant level was determined as P<0.05 using unpaired students’ test. **, P < 0.01.