Research Paper Volume 14, Issue 16 pp 6668—6688
MicroRNA-148a induces apoptosis and prevents angiogenesis with bevacizumab in colon cancer through direct inhibition of ROCK1/c-Met via HIF-1α under hypoxia
Figure 5. miR-148a suppresses VEGF secretion and the angiogenesis of bevacizumab in HCT116 colon cancer cells under hypoxic conditions. (A) Human umbilical vein endothelial cell tube formation assay was performed in four HCT116 cell lines (HCT116, HCT116 + bevacizumab, HCT116-miR-148a, and HCT116-miR-148a + bevacizumab); (B) Both miR-148a and bevacizumab significantly inhibited human umbilical vein endothelial cell formation (P < 0.001 and P = 0.001; respectively); (C) VEGF expression levels in HCT116, HCT116 + bevacizumab, HCT116-miR-148a, HCT116-miR-148a + bevacizumab cells obtained using Western blotting under hypoxia; (D) miR-148a significantly inhibited VEGF secretion as shown in Western blotting analysis (P = 0.007) but not in HCT116 + bevacizumab cells. β-Actin served as an internal control.