Research Paper Volume 14, Issue 16 pp 6520—6536
Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells
Figure 4. Overexpression of Dicer reduced enhances the migration, invasion, and stemness of breast cancer cells. (A) Western blot analysis of Dicer overexpression in highly invasive (MCF-7/I-3 and MDA-MB-231/I-3) cells. (B) The migration and invasion of the indicated cells were examined using the cell migration and invasion assays, respectively. (C) Flow cytometry of ALDH activity in Dicer-overexpressing breast cancer cells. (D) Self-renewal ability was examined in Dicer-overexpressing breast cancer cells by using the sphere formation assay. Data are presented as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.