Research Paper Volume 14, Issue 15 pp 6128—6148

Figure 3. Upregulation of αB-crystallin in Mab21L1-αTN4-1 cells and its attenuated degradation during okadaic acid (OA)-induced apoptosis of the Mab21L1-αTN4-1 cells. Both vector-αTN4-1 and MAB21L1-αTN4-1 cells were grown to about 90% confluence and then subjected to 100 nM OA treatment for 12 and 24 hrs. Thereafter, the cells were harvested for extraction of total proteins which were used for analysis of αB-crystallin expression by Western blot analysis (A). Quantitative results of the αB-crystallin protein expression levels were analyzed by Image J software (B). Note that in the MAB21L1-αTN4-1 cell clones, the αB-crystallin protein expression level was much higher than that in the vector-αTN4-1 cell clone in the absence of 100 nM OA treatment. During OA treatment for 12 and 24 hrs, the degradation of αB-crystallin protein was much slower in MAB21L1-αTN4-1 cell clones than that in vector-αTN4-1 clone. N=3. ** p<0.01.