Research Paper Volume 14, Issue 14 pp 5878—5894

Figure 9. MBD1 and HDAC3 directly bound to the promoter region and inhibited the expression of miR-5701. (A) The binding sites of MBD1, HDAC3 and DNMT3A in the promoter of miR-5701. (B) ChIP-PCR showed that DNMA3A, HDAC3 and MBD1 directly bound to the promoter of miR-5701. (C) Immunofluorescence staining showed that MBD1 and HDAC3 co-localized in nuclei of SGC-7901 cells. (D) IP assay showed that MBD1 directly bound to HDAC3 at the protein level. (E) SGC-7901 cells were treated with siMBD1 or siHDAC3 and the expression of miR-5701 was measured by qRT-PCR. Data are represented as the mean±SD and experiments were performed in triplicate; *p<0.05, **p<0.01**.