Research Paper Volume 14, Issue 14 pp 5878—5894

Figure 6. FGFR2 functions in MKN-45 cells. MKN-45 cells were transfected with FGFR2 overexpressed plasmid and (A) Cell viability was measured by MTT assay at the points of 24h, 48h and 72h; (B, C) Cell proliferation was measured by colony formation assay; (D–G) Cell migration was measured by transwell assay and scratch assay, and migration associated proteins were measured by western blot. (H, I) Cell apoptosis rates were measured by flow cytometry and apoptosis associated proteins were measured by Western blot. MKN-45 cells were transfected with FGFR2 overexpressed plasmid or its control, at the same time were infected with miR-5701 or its control, and (J) Cell viability were measured by MTT assay at the points of 24h, 48h and 72h; (K) Cell apoptosis rates were measured by flow cytometry at 48h.