Research Paper Volume 14, Issue 14 pp 5878—5894

Figure 2. Expression and function of miR-5701 in GC cell line SGC-7901. (A) The expression of miR-5701 in GC tissues versus normal gastric tissues. (B) The expression of miR-5701 in GC cell lines. GES-1 was used as control. SGC-7901 cells were infected with miR-5701 or were treated with ASO-miR-5701. (C) Cell viability was measured by MTT assay at the time of 24h, 48h and 72h. (D, E) Cell proliferation was measured by colony formation assay; (F, G) Cell apoptosis was checked by flow cytometry; (H) Apoptosis-associated proteins were measured by Western blot; (I–L) Cell migration were measured by scratch assay and transwell assay, and migration associated proteins were measured by Western blot. Data are represented as the mean±SD and experiments were performed in triplicate; * p<0.05; ** p<0.01.