Research Paper Volume 14, Issue 12 pp 5250—5270

Figure 1. Expression and prognostic value of TRPM7 mRNA for HNSCC based on the TSGH and TCGA cohort. (A) Heatmap of TRPM7 expression in Tri-Service General Hospital (TSGH) patients with head and neck squamous cell carcinoma. (B) Immunostaining showed that TRPM7 expression in the tissues of HNSCC patients was higher than that in normal tissues. (C) Box-plot diagrams of TRPM7 and NFATC3 expression in TCGA. (D) Using the GSE26549 dataset (n = 44), we demonstrated that high TRPM7 mRNA expression is associated with worse 5-year overall survival compared with low TRPM7 mRNA expression. (D) Overall survival of HNSCC patients with a high NFATC2 expression had a poor overall survival rate from the TCGA cohort (E) A positive correlation was observed between the expression of the calcineurin/NFAT pathway-related targets NFATC3 and TRPM. (F) Expression analysis of TRPM7 and NFATC3 with tumor stage from the TCGA cohort (Image generated from: http://gepia2.cancer-pku.cn/#analysis). (G) Immunofluorescence assay to show the co-localization of TRPM7 and calcineurin/NFAT in SAS cells. *p < 0.05, **p < 0.01, ***p < 0.001.