Research Paper Volume 14, Issue 12 pp 5211—5222
DNA methylation-regulated SNX20 overexpression correlates with poor prognosis, immune cell infiltration, and low-grade glioma progression
Figure 10. Depletion of SNX20 inhibits GBM cell proliferation and migration. (A) The expression of SNX20 in normal human astrocytes cells (NHA) and GBM cell lines (U87, A172 and U251). (B) The SNX20 knockdown efficiency in A172 and U87 cells were verified by qRT-PCR assay. (C–E) SNX20 knockdown significantly inhibited A172 and U87 cells proliferation examined by growth curve and colony formation assays. (F) SNX20 knockdown significantly inhibited A172 and U87 cells migration examined by transwell assay. Scale bar=50 μm. NC=Negative control, siRNA= SNX20 siRNA, * P < 0.05, ** P < 0.01, *** P < 0.001.