Research Paper Volume 14, Issue 16 pp 6507—6519

Figure 5. miR-141 inhibits EGFR to downregulate β-catenin, thereby enhancing autophagy to promote lung I/R injury in vivo. (A) RT-qPCR was used to detect the expression of miR-141 and EGFR in mouse lung tissues. (B) Western blotting was used to detect the protein expression of ERFG, β-catenin, LC3II/I and BECN1 in mouse lung tissues. (C) Blood gas analyzer was used to detect the blood gas in arterial blood in left ventricle of mice. (D) The statistical graph of W/D ratio of lung tissues of mice. (E) HE staining results of lung tissues (× 400) and lung injury scores. (F) TUNEL staining (× 200) was used to detect the apoptosis of mouse lung cells. * p < 0.05 was considered statistically significant. The experiment was repeated three times independently. Results were expressed as the mean ± SD.