Figure 4.miR-141/EFGR/β-catenin axis gives rise to autophagy, contributing to the progression of H/R-induced injury in vitro. (A) miR-141 and EGFR expression in mouse PMVECs was measured by RT-qPCR. (B) EGFR, β-catenin, LC3II/I and BECN1 expression in mouse PMVECs were measured by Western blot analysis. (C) LC3 immunofluorescence assay (× 400) was used to detect autophagosomes of PMVECs. (D) the apoptosis rate of PMVECs was measured by flow cytometry assay. * p < 0.05 was considered statistically significant. The experiment was repeated three times independently. Results were expressed as the mean ± SD.
