Research Paper Volume 14, Issue 16 pp 6507—6519

Figure 2. miR-141 inhibition suppresses autophagy and relieves lung I/R injury in vivo and in vitro. (A) RT-qPCR analysis for analyzing miR-141 levels within lung tissues of IRI mice. (B) Western blotting was used to detect the protein expression of LC3II/I and BECN1 in mouse lung tissues. (C) Blood gas analyzer was used to detect the blood gas in arterial blood in left ventricle of mice. (D) The statistical graph of W/D ratio of lung tissues of mice. (E) HE staining results of lung tissues (× 400) and lung injury scores. (F) TUNEL staining (× 200) was used to detect the apoptosis of mouse lung cells. (G) RT-qPCR analysis for analyzing the miR-141 expression in mouse PMVECs. (H) Western blotting was used to detect the protein expression of LC3II/I and BECN1 in mouse PMVECs. (I) LC3 immunofluorescence assay was used to detect autophagosomes. (J) flow cytometry assay was used to detect the apoptosis of PMVECs. * p < 0.05 was considered statistically significant. The experiment was repeated three times independently. Results were expressed as the mean ± SD.