Research Paper Volume 14, Issue 14 pp 5669—5680

Figure 1. Effect of KLF4 overexpression on M1 polarization of macrophages. (A, B) The proportion of M1 cells was detected by flow cytometry (n=3): KLF4 overexpression (pEGFP-KLF4) promoted the M1 polarization of macrophages and increased the F4/80+CD86+ cell proportion. **P<0.01 between groups. (C) IF staining of CD86 expression (n=3). KLF4 overexpression (pEGFP-KLF4) promoted CD86 expression and increased the fluorescence intensity. (D) Expression of M1 cell marker proteins (n=3, TNF-α, IL-6, IL-1β). KLF4 overexpression (pEGFP-KLF4) up-regulated the expression of inflammatory factors TNF-α, IL-6 and IL-1β. **P<0.01 between groups. (E) Expression of mRNA (n=3, TNF-α, IL-6, IL-1β). KLF4 overexpression (pEGFP-KLF4) up-regulated the expression of mRNA. **P<0.01 between groups. (F, G) Expression of JAK1-STAT1 signal proteins (n=3). KLF4 overexpression promoted the expression of JAK1 and STAT1 proteins, increased their phosphorylation levels. **P<0.01, relative protein expression between groups.