Research Paper Volume 14, Issue 14 pp 5669—5680

Transcription factor KLF4 regulated STAT1 to promote M1 polarization of macrophages in rheumatoid arthritis

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Figure 1. Effect of KLF4 overexpression on M1 polarization of macrophages. (A, B) The proportion of M1 cells was detected by flow cytometry (n=3): KLF4 overexpression (pEGFP-KLF4) promoted the M1 polarization of macrophages and increased the F4/80+CD86+ cell proportion. **P<0.01 between groups. (C) IF staining of CD86 expression (n=3). KLF4 overexpression (pEGFP-KLF4) promoted CD86 expression and increased the fluorescence intensity. (D) Expression of M1 cell marker proteins (n=3, TNF-α, IL-6, IL-1β). KLF4 overexpression (pEGFP-KLF4) up-regulated the expression of inflammatory factors TNF-α, IL-6 and IL-1β. **P<0.01 between groups. (E) Expression of mRNA (n=3, TNF-α, IL-6, IL-1β). KLF4 overexpression (pEGFP-KLF4) up-regulated the expression of mRNA. **P<0.01 between groups. (F, G) Expression of JAK1-STAT1 signal proteins (n=3). KLF4 overexpression promoted the expression of JAK1 and STAT1 proteins, increased their phosphorylation levels. **P<0.01, relative protein expression between groups.