Research Paper Volume 14, Issue 12 pp 5059—5074

Figure 3. mmu_circ_0015268 contributed to lung inflammation and vascular injury. Adult C57BL/6 mice were randomly assigned into 6 groups: sham, Model, Model + empty vector (EV), Model + mmu_circ_0015268 overexpressing vector (OV), Model + negative control (NC), Model + mmu_circ_0015268 shRNA (shRNA). ELISA was used to detect the contents of TNF-α, IL-6, and IL-1β in whole lung tissues (A) and BAL fluid (B). (C) Paraffin-embedded lung tissue samples were stained for H&E (magnification 200 X). (D) TUNEL assay of lung tissue sections for evaluation of apoptosis (magnification 200 X). (E, F) Quantification of lung injury scores and apoptosis. *P < 0.05; **P < 0.01 vs. sham; #P < 0.05, vs. model.