Research Paper Volume 14, Issue 11 pp 4819—4826
NEAT1 variant 1 weakens the genome-wide effect of miR-3122 on blocking H3K79me3 in bladder cancer
Figure 4. miR-3122 enriches the histone H3 with robust H3K79me3 modification. (A) Representative MS2 spectra shows H3 protein in T24 cells. Inset: Fragmentation patterns of b and y ions show sequence information, amino acid residue, m/z and charge state identified by LC-MS/MS. (B) RIP-qPCR assay shows the enrichment of miR-3122 in histone 3 with different lysine methylation in T24, BIU-87 and EJ-1 BC cells. The p-values represent the statistical significance compared with K3K79me3 or IgG according to different color fonts via One-way ANOVA analysis.