Figure 8. Effects of pLXSN and WT-TP53 on clonogenicity in the presence of 5-Fluorouracil, gemcitabine or cisplatin in two PDAC cell lines. The clonogenicity in the presence of increasing concentrations of 5-fluorouracil (5FU), gemcitabine (Gem) and cisplatin (Cis) were examined in: MIA-PaCa-2 + pLXSN and MIA-PaCa-2 + WT-TP53 (A–C), PANC-28 + pLXSN, and PANC-28 + WT-TP53 (D–F). Red horizontal bars = MIA-PaCa-2 or PANC-28 containing pLXSN. Blue horizontal bars = MIA-PaCa-2 or PANC-28 containing WT-TP53. These experiments were repeated and similar results were observed. The colonies for each cell line were normalized to untreated so that the results from pLXSN and WT-TP53 could be compared. ***P < 0.0001, **P < 0.005 and *P < 0.05.