Research Paper Volume 14, Issue 8 pp 3666—3686
LncRNA136131 suppresses apoptosis of renal tubular epithelial cells in acute kidney injury by targeting the miR-378a-3p/Rab10 axis
Figure 2. lncRNA136131 knock down aggravated the I/R-induced BUMPT cells apoptosis and the expression of cleaved-caspase3. BUMPT cells were transfected with 100 nM lncRNA136131 siRNA or scramble, and then treated with or without I(2 hours)/R(2 hours) injury. (A) RT-qPCR analysis of the expression of lncRNA136131. (B) FCM analysis of BUMPT cells apoptosis. (C) Analysis of apoptosis rate (%). (D) The immunoblot analysis of caspase 3, cleaved-caspase3, Bax, p53 and Blc-2. (E) The gray analysis between them. Data are expressed as mean ± SD (n = 6). *p < 0.05, I/R with scramble or lncRNA136131 siRNA group versus scramble group; #p < 0.05, I/R with lncRNA136131 siRNA group versus I/R with scramble group.