Research Paper Volume 14, Issue 8 pp 3617—3632
Puerarin protects against sepsis-induced myocardial injury through AMPK-mediated ferroptosis signaling
Figure 5. Puerarin suppresses LPS-induced myocardial ferroptosis via up-regulation the phosphorylation of AMPK. (A, B) Representative Western blot analysis and quantitative protein analysis of GPX4 and ACSL4 expression in cardiac protein extract. (C, D) Representative Western blot analysis and quantitative protein analysis of Ferritin and TFR expression in cardiac protein extract. (E, F) Representative Western blot analysis and quantitative protein analysis of P-AMPK expression in cardiac protein extract. (G) represents the amount of iron in the heart. Data represent the mean ± SD. *P < 0.05 vs Control group; #P < 0.05 vs. LPS group; &P < 0.05 vs. LPS+Pue group.