Research Paper Volume 14, Issue 6 pp 2793—2804
Local anesthetic levobupivacaine inhibits stemness of osteosarcoma cells by epigenetically repressing MAFB though reducing KAT5 expression
Figure 4. Levobupivacaine epigenetically inhibits MAFB expression by reducing KAT5 in osteosarcoma cells. (A–C) MG63 and U2OS cells were treated with levobupivacaine (2 mM). The expression of MAFB and KAT5 was detected by qPCR and Western blot analysis. (C) The enrichment of KAT5 on MAFB promoter was analyzed by ChIP analysis. (D–F) MG63 and U2OS cells were transfected with KAT5 siRNA. (D) The enrichment of H3K27ac on MAFB promoter was determined by ChIP analysis. (E) The enrichment of RNA polymerase II on MAFB promoter was tested by ChIP analysis. (F) MAFB expression was assessed by qPCR and Western blot analysis. (G) MAFB expression was examined by Western bot in MG63 and U2OS cells treated with levobupivacaine, or co-treated with levobupivacaine and KAT5 overexpression vector. mean ± SD, ** P < 0.01.