Research Paper Volume 14, Issue 6 pp 2678—2694

Figure 3. piRNA-6426 interacts with DNMT3B. (A) Immunoprecipitation method of piRNA-6426 binding to DNMT3B in rat cardiomyocytes. The cells were incubated with LV-piRNA-6426 or sh-piRNA-6426 for 48 h. (B) RT-qPCR assay was used to detect the expression of piRNA-6426 in cardiomyocytes incubated with LV-piRNA-6426 or empty vector. (C) Western blotting was used to detect the expression level of DNMT3B protein LV-piRNA-6426 in cardiomyocytes incubated with LV-piRNA-6426 or empty vector. (D) RT-qPCR assay was used to detect the expression of piRNA-6426 in cardiomyocytes incubated with sh-piRNA-6426 or scrambled shRNA. (E) Western blotting was used to detect the expression level of DNMT3B protein LV-piRNA-6426 in cardiomyocytes incubated with sh-piRNA-6426 or scrambled shRNA. Values were expressed as mean ± SEM. ns P>0.05, *P<0.05, **P<0.01, n=6.