Research Paper Volume 14, Issue 6 pp 2678—2694

Figure 2. Overexpression of piRNA-6426 alleviates hypoxia-induced dysfunction of rat cardiomyocytes. The isolated and cultured rat cardiomyocytes were induced in a hypoxic incubator for 24 hours to establish a HF cell model after 24 h of per-incubated with 25 nM piRNA-6426 overexpression vector. (A) The expression of piRNA-6426 in each group were detected by using RT-qPCR. (B) MTT assay was used to identify cell viability. (C, D) The production of reactive oxygen species (ROS) was analyzed with DCFH-DA. (E, F) Flow cytometry was used to detect cell apoptosis. (G) The lactate dehydrogenase (LDH) activity was detected. (H) D-(2-3H)-glucose uptake assay was used to perform glucose uptake on fully fused rat cardiomyocytes. (I, J) ELISA kits were used to detect the secretion of inflammatory cytokines IL-1β and TNF-α. Values were expressed as mean ± SEM. *P<0.05, **P<0.01, n=6.