Research Paper Volume 13, Issue 22 pp 24753—24767

Figure 1. Fisetin inhibits the proliferation of PANC-1 cells. (A) PANC-1 cells were inoculated with Astragaline, Afzelin, Quercetin 3-O-α-L-arabinoside, Narcissoside, Avicularin, Fisetin, Herbacetin, 3'-Hydroxyflavanone and Kaempferol-3-O-rutinoside (50 μM), and the cell index was recorded by RTCA. (B) Histogram exhibiting the repression rate of different compounds. (C) Colony formation assessment of PANC-1 cells inoculated with 0,50 and 100 μM fisetin. (D) Histogram illustrating the number of colonies in each group. (E) Ki67 Immunofluorescence staining of PANC-1 cells inoculated with 0,50 and 100 μM fisetin for 24h. (F) Histogram illustrating the Ki67 positive rate of PANC-1 cells in each group. All assays were replicated thrice, and data are given as means±SD.*p<.05,**p<.01,***p<.001, in contrast with the controls.