Research Paper Volume 13, Issue 22 pp 24740—24752

Figure 2. Effects of Fam114A1 on the function of melanocytes. (A, B) Western blot were used to detect the expression of Fam114A1 protein in MC after overexpression and suppression of plasmid transfection. The results showed that the expression of Fam114A1 protein in MC was successfully suppressed and overexpressed, Statistical analyses were performed using one-way ANOVA and Dunnett’s post hoc test: **P < 0.01 vs Control. (C, D) The effect of Fam114A1 on MC apoptosis was analyzed by mitochondrial membrane spot detection. It can be seen that when Fam114A1 protein is overexpressed, the apoptotic rate of MC increases significantly, and when the expression is inhibited, the apoptosis rate is dropped significantly. Statistical analyses were performed using one-way ANOVA and Dunnett’s post hoc test: *P < 0.05 vs Control. (E, F) Western blot was used to detect the expression of apoptosis-related proteins. It can be seen that the expression of apoptotic proteins bax, caspase3, cleaved-caspase3, and P53 decreased significantly in MCs inhibited by Fam114A1 protein, while the expression of apoptosis inhibitor bcl2 was significantly increased. Statistical analyses were performed using one-way ANOVA and Dunnett’s post hoc test: *P < 0.05 vs Control. (G, H) The migration of melanocyte was confirmed with transwell. activity. The migration of melanocyte was inhibited in sh-Fam MC compared with its control, Statistical analyses were performed using one-way ANOVA and Dunnett’s post hoc test: *P < 0.05 vs Control. (I) The expression of melanin synthesis proteins was detected by RT-PCR, the decrease of TYR and MITF was observed in sh-Fam MC vs its control, Statistical analyses were performed using one-way ANOVA and Dunnett’s post hoc test: *P < 0.05 vs Control.