Research Paper Volume 13, Issue 19 pp 23308—23327
Transcriptome and proteome analysis of the antitumor activity of maslinic acid against pancreatic cancer cells
Figure 9. Overexpression of UACA and AK4 abolishes the MA-mediated anti-tumor effect. (A) QRT-PCR and western blot analysis of the expression of UACA and AK4 in NC, oe-UACA, oe-AK4 groups of PANC-1 cells. (B) Colony formation assay analysis of cell proliferation in NC, NC+MA (40μM MA treated for 24 h), oe-UACA+MA (40μM MA treated for 24 h), oe-AK4+MA (40μM MA treated for 24 h), oe-UACA, oe-AK4 groups of PANC-1 cells. (C) Wound healing assay analysis of cell migration in NC, oe-UACA+MA (40μM MA treated for 24 h), oe-AK4+MA (40μM MA treated for 24 h), oe-UACA, oe-AK4 groups of PANC-1 cells. Bar =200 μm. Data are presented as fold change and as mean ± SD of three independent experiments. *p <0.05, **p < 0.01, ****p < 0.0001.