Research Paper Volume 13, Issue 19 pp 23133—23148

Figure 4. Effect of HFD on activation of astrocytes and microglia in the mice cortex and cerebellum. (A) Western blots and (B–F) densitometric analysis for IL-1β 31 kDa (B), IL-1β 17 kDa (C), GFAP (D), Iba-1 (E), vimentin (F) after HFD in mice cerebral cortex at day 7, day 14 and day 21 (n = 3 to 4 per group). (G) Immunohistochemistry of GFAP⁺ astrocytes and Iba-1⁺ microglia in cerebral cortex tissue sections. High magnification images of astrocytes and microglia in a larger box outlined by red color indicated by arrows. Magnification, ×40. Scale bars, 10 μm. (H) Western blot analysis of cerebellum tissues of mice with HFD and chow diet controls (n = 3 per group). (I–M) Quantification of IL-1β 31 kDa (I), IL-1β 17 kDa (J), Iba-1 (K), GFAP (L) and vimentin (M).Vinculin and β-actin as a loading control. Values are presented as means ± SD. ^*P < 0.05 and ^**P < 0.01 versus chow diet; one-way ANOVA by Tukey’s test for cerebral cortex. Two-tailed Student’s t test for cerebellum.