Research Paper
Silencing of long noncoding RNA MAFG-AS1 prevents autophagy and epithelial-mesenchymal transition via miR-24-3p/ B4GAT1 in ovarian cancer
Figure 3. MiR-24-3p is downstream target of MAFG-AS1. (A) The level of MAFG-AS1 in cytoplasm and nucleus. (B) Bioinformatics website predicted the target of MAFG-AS1. (C) The level of miR-24-3p, miR-143-3p, and miR-147b in SKOV3 after siMAFG-AS1 transfection. n = 6, *P<0.05. (D) MiR-24-3p expression in OC cell lines was quantified by qRT-PCR. n = 4, *P<0.05. (E) The relationship between MAFG-AS1 and miR-24-3p. (F) Luciferase reporter assay was used to determine the luciferase activity in HEK-293T cells of indicated groups. n = 3, *P<0.05. (G) The enrichment of miR-24-3p and MAFG-AS1 in AGO2 and IgG detected by RIP assay. n = 3, *P<0.05. (H) Biotin-RNA pull-down assay. n = 3, *P<0.05. (I) FISH assay.