Research Paper

Figure 2. Silencing of MAFG-AS1 prevents cell development in OC cells. (A) The knockdown efficiency of siRNA was confirmed by qRT-PCR. n = 4, *P<0.05. (B) The cell viability of MAFG-AS1 on OC cells was detected by CCK-8 assay. n = 6, *P<0.05. (C) Clone formation assay was used to detect proliferation. n = 4, *P<0.05. (D) The proliferative ability of OC cells transfected with MAFG-AS1 siRNA was assessed by EdU staining assay. (E) Pictures of tumor spheres formed by OC cells and the quantity of sphere formation efficiency. Scale bar: 100 μm. (F) The migration and invasion of MAFG-AS1 knockdown OC cells were measured by Transwell assay. n = 4, *P<0.05. (G) The protein level of Bcl1, Bax, and Cytochrome c was detected by Western blot. n = 4, *P<0.05. (H) The protein level of E-cadherin, N-cadherin, MMP2, MMP7 was detected by Western blot. n = 4, *P<0.05. (I) The protein level of LC3, P62, LAMP1 was detected by Western blot. n = 4, *P<0.05.