Research Paper Volume 14, Issue 5 pp 2210—2220

Figure 5. MiR-214-3p/PAK6 may be downstream of circ_0001006. (A) Bioinformatics website forecast revealed that circ_0001006 includes site binding to miR-214-3p provided by predication tools (http://starbase.sysu.edu.cn/index.php). (B) circ_0001006 in cell lysis was pulled down and collected with a circ_0001006-specific probe and then measured using qRT-PCR. miR-214-3p was pulled down and collected with a circ_0001006-specific probe and then evaluated using qRT-PCR. (C) Luciferase assay report verified the relationship between circ_0001006 and miR-214-3p. (D and E). the expression of miR-214-3p in cardiomyocytes after sh-circRNA and circRNA transfection. (F) Luciferase assay report verified the relationship between PAK6 and miR-214-3p. (G–K). The expression of PAK6 was detected in cardiomyocytes. Data are shown as mean ± SEM. ^*P < 0.05 and ^**P < 0.01. All experiments were repeated at least three independent experiments.