Research Paper Volume 14, Issue 5 pp 2210—2220

Figure 2. circ_0001006 was upregulated in AngII-induced cardiomyocyte hypertrophy. (A) The immunofluorescence results of cardiomyocytes treated with or without Ang II. (α-actinin, red, DAPI, blue, The scale bar is 25 μm). (B) qRT-PCR assay analysis of the circ_0001006 expression level in cardiomyocytes. (C) Western blot analysis of ANP, BNP, β-MHC expression in cardiomyocytes. (D) Protein/DNA ratio of the cardiomyocytes. (E) The expression of circ_0001006 in vitro. ^*P < 0.05 and ^**P < 0.01. Data are shown as mean ± SEM. All experiments were repeated at least three independent experiments.