Figure 3.Overexpression of NPAS2 inhibited autophagy in ischaemia/reperfusion injury rats. (A) The protein level of NPAS2 in rat myocardial tissue was determined using Western Blot. (B) The protein level of Cleaved-Caspase-3 (17kDa) and Caspase-3 17kDa) in rat myocardial tissue was determined by Western Blot. (C) The protein level of LC3B and p62 in rat myocardial tissue was determined using Western Blot. (D) Representative photomicrographs of LC3B (green) immunofluorescence in rat myocardial tissue. DAPI was used to counterstain nuclei.
