Research Paper Volume 13, Issue 16 pp 20534—20551
NLRP3 inflammasome activation contributes to the pathogenesis of cardiocytes aging
Figure 9. NAC decreased ROS generation in the cardiocytes aging model. H9c2 cells were pre-treated with or without NAC (1 mM), a commonly used ROS scavenger, for 1 hour, and then incubated with or without 10 g/L D-gal for 24 hours. (A) ROS generation was detected using a DCFH-DA probe. Representative fluorescent images showed that NAC decreased ROS generation in the cardiocytes aging model induced by D-gal in vitro. (B) Intracellular ROS was quantified by flow cytometry.