Figure 8. Quantitative analysis of cGMP in aortas from young and aged wild-type (WT) littermates aortas (A) and eAPP−/− mice aortas (B) under basal and ACh (10 μM) stimulated conditions. All results were normalized against tissue protein levels and are representing box plots with whiskers showing the median, 25th to 75th percentiles, and min-max range (n=13 per group for young WT littermates and eAPP−/− mice and n=10 per group for aged WT littermates and eAPP−/− mice). * P<0.05 versus basal levels; † P<0.05 versus young WT mice (two-way ANOVA followed by Tukey’s HSD test).